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Polymers for fluorescence imaging in infectiology

Disciplines Biology, Chemistry, Physics
Research fields Imaging, Virology
Supporting organisms CNRS, ENS de Lyon
Geographical location
ENS de Lyon (Campus Charles Mérieux)
Lab
LJC
Team leader
Marie-Thérèse Charreyre
Webpage
http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique25


Our project in Joliot-Curie Laboratory is dedicated to fluorescence imaging, more precisely to the design of original bio-imaging probes combining multifunctional polymers and chromophores, and to their application to reply to three specific biological questions :

  • the assembly and spreading of viral particles from the host cell,
  • the multi-labelling of various phagocyte sub-populations,
  • the study of new apoptosis pathways


The specific contribution of multifunctional polymers to the bio-imaging probes is based on immobilization of recognition moieties, labelling moieties and possibly therapeutic or cytotoxic moieties along the chain. The project objective appears relevant for many applications including ex vivo imaging of cells and in vivo diagnostic possibly combined with a local therapy (theranostic). It is based on a multidisciplinary approach that associates chemistry (molecular and macromolecular), spectrophysics and biology (virology and immuno-apoptosis).
This project is strongly based on our expertise in macromolecular chemistry applied to biology that includes several approaches :

  • kinetics and mechanistic studies of so-called “living” polymerization techniques (especially the RAFT process) providing very well-defined polymer chains.
  • synthesis of several kinds of macromolecular architectures, such as random copolymers, block copolymers, graft copolymers, end-functional copolymers (including reactive copolymers, glycopolymers and fluorescent polymers).
  • elaboration of polymer/biomolecule and polymer/chromophore conjugates via immobilization of biomolecules and chromophores by covalent bonds onto the various polymer architectures.